Description
The Across Pro family of pipettes incorporates multiple features that bring comfort and efficiency to the repetitive but essential liquid handling process. OHAUS’ newest generation of adjustable, single channel precision pipettes includes eight models that can hold volumes ranging from 0.2 μL to 10 mL and support every application in which precise measurement of liquids is a main priority. Micropipette in Bangladesh
| Applications | Pipetting |
|---|---|
| Display | 3-digit micrometer |
| Operation | N/A Micropipette in Bangladesh |
| Communication | N/A Micropipette in Bangladesh |
| Construction | PVDF housing, stainless steel piston, ergonomic finger hook |
| Design Features | Highly chemical and shock resistant, low thermal conductivity, light plunger force, low-drag seals |
Micropipette in Bangladesh
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Micropipette Means
A micropipette is a common yet an essential laboratory instrument used to accurately and precisely transfer volumes of liquid in the microliter range. Micropipettes are available in single channel and multi channel variants. While the single channel micropipettes are used in labs that perform research related to molecular biology, microbiology, immunology, cell culture, analytical chemistry, biochemistry and genetics, the multichannel micropipettes are recommended for ELISA (diagnostic test), molecular screening, kinetic studies and DNA amplification.
Component Micropipette in Bangladesh
Micropipettes are available in different designs and sizes. However, there are certain components that are basic and common to all the micropipettes. These include the plunger, digital display, tip cone, tip ejector and grippy. Certain micropipettes are provided with a calibration tool and a micropipette stand as an accessory.
Plunger: The plunger performs the following two functions- Micropipette in Bangladesh
Volume adjustment: Rotate the plunger clockwise/ anticlockwise to decrease/ increase the volume setting. A distinct click sound at every volume change ensures perfect volume setting and prevents any accidental volume change.
Liquid aspiration/ dispensing: Press and depress the plunger to aspirate or dispense liquid.
Tip Ejector: The internal mechanism of the micropipette does not come in direct contact of the sample/liquid. Instead, a disposable pipette tip is used to draw the liquid into and dispense from the micropipette. So in order to allow the safe, effortless and quick ejection of tips, micropipettes are provided with a tip ejection system. The tips can be easily removed from the micropipette by pressing the tip ejector button.
Volume Display: This shows the volume of the liquid to be aspirated or dispensed.
Tip Cone: The tip cone provides fitment to the tips. A micropipette with a universal tip cone is preferred as it enhances the compatibility of the instrument with most of the standard tips.
Micropipette Type:
Micropipettes can be classified depending upon:
Working Principle: The plunger performs the following two functions-
Air Displacement Micropipette: This type of micropipette works on the air displacement principle. It consists of a piston which aspirates and dispenses liquid samples as the air pocket moves up and down, respectively. The internal mechanism of the pipette does not come in direct contact of the sample/liquid. Instead, a disposable pipette tip is used to draw the liquid into and dispense from the pipette.
Positive Displacement Micropipette: In these micropipettes, the piston comes in direct contact of the sample. The disposable tip in a positive displacement micropipette is a microsyringe composed of a capillary and a piston (movable inner part) which directly displaces the liquid.
Number of Channel:
Single Channel Micropipette: A single channel micropipette is the one which has a single channel to aspirate or dispense the liquid.
Multi Channel Micropipette: A multi channel micropipette has multiple channels to aspirate or dispense the liquid. The commonly available multi channel micropipette variants are the 8 channel, 12 channel and 16 channel. Multichannel micropipettes reduce the workload of a single channel micropipette when working with large volumes of samples.
Volume / Capacity:
Fixed Volume Micropipette: In a fixed volume micropipette, the volume of liquid to be aspirated or dispensed remains fixed. These micropipettes are used when the same volume of liquid is to be dispensed multiple times.
Variable Volume Micropipette: This micropipette comes with a specific minimum and maximum volume range. The volume of the liquid to be aspirated or dispensed can be adjusted (within the instrument’s volume range) depending upon the requirement of the user.
Micropipette work
Air displacement micropipettes operate by piston-driven air displacement. When the piston is pressed downwards, the air within the sleeve of the micropipette gets expelled out due to the force of which the liquid present in the micropipettes tip also gets removed. And. So so. And. So so. And. So so
Positive displacement micropipettes operate by piston-driven displacement. The piston in a positive displacement micropipette is in direct contact with the liquid. When the piston is pressed downwards, the liquid which is present in the sleeve of the micropipette also moves downwards and gets removed through the tip. When the piston is pulled upwards, it also draws the liquid along with it in the upward direction. And. So so. And. So so. And. So so
Air Displacement Micropipette in Bangladesh
Precise measurement of liquid depends on the correct micropipette usage. The air displacement micropipettes work on the common air displacement principle. In between these steps, there are several small steps which help in making the liquid dispensing process more precise. And. So so. And. So so. And. So so
Some micropipettes deliver the fixed volume of liquid. However, the majority are adjustable with the variable volume setting. Variable volume micropipette comes with different ranges and upper & lower limits of measurement. In such cases, error percentage may vary as per the measured liquid. Trying to dispense less than the lower value of the range will result in inaccurate liquid measurements where trying to dispense over the upper range will completely fill the tip and allow the liquid to enter into the pipette body. Trying to dispense less than the lower value of the range will result in inaccurate liquid measurements where trying to dispense over the upper range will completely fill the tip and allow the liquid to enter into the pipette body. And. So so. And. So so. And. So so
Cleaning
The care and maintenance of a micropipette is an important routine in laboratories. Implementing a proper maintenance schedule can reduce the cost of a new purchase of this expensive equipment. So, it is important to handle the micropipette carefully while cleaning. The following is a comprehensive guide for proper cleaning of micropipettes. Cleaning the micropipette takes time. Focus and practice; otherwise the micropipette can be damaged. So, it is important to handle the micropipette carefully while cleaning. The following is a comprehensive guide for proper cleaning of micropipettes. And. So so. And. So so. And. So so
External Cleaning: Most of the pipette can be cleaned externally with typical laboratory cleaning agents, soaps or alcohol. To ensure the full sterilization, let the cleaning solution sit on the micropipette for 10-15 min before wiping it off. And. So so. And. So so. And. So so
Internal Cleaning: Cleaning the interior of the micropipette can be more time consuming because it requires full disassembly. Also, every part of micropipette will need to be cleaned properly depending on the liquid used in sampling. And. So so. And. So so. And. So so
- Refer to the instructions manual for specific direction of micropipette. And. So so. And. So so. And. So so
- Use a cotton swab with cleaning solution and distilled water. And. So so. And. So so. And. So so
- Lightly grease the pistons with the lubricant provided upon purchase. And. So so. And. So so. And. So so
- Reassemble all parts and check to ensure the micropipette operates smoothly. And. So so. And. So so. And. So so
Contamination Cleaning:
If micropipette becomes contaminated with a known substance. There are specific cleaning steps that must be taken depending on the type of substance, Above cleaning routine will not be sufficient if the micropipette is cross-contaminated. And. So so. And. So so. And. So so
A well-maintained and clean pipette ensures the lab safety from hazardous solutions. It makes the pipette more accurate, reliable, long-lasting and reduces the cost of sampling. And. So so. And. So so. And. So so
Proper storage of micropipettes is as important as cleaning and calibrating them. Cool and dry place. The storage place should have a temperature ranging from -20 °C to 50 °C (from -4 °F to 120 °F) with relative humidity between 5% and 95%. To store pipettes, Microlit recommends the use of its carousel stand, Microlit Faveo.
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