Ohaus Pipette (20 – 200 µL)

Description

We are Ohaus Pipette Supplier in Bangladesh. Aside from sending your pipette off for calibration every year, your pipettes should be checked for accuracy on a regular basis. How often you do it depends on the frequency you use them.

The Across Pro family of pipettes incorporates multiple features that bring comfort and efficiency to the repetitive but essential liquid handling process. OHAUS’ newest generation of adjustable, single channel precision pipettes includes eight models that can hold volumes ranging from 0.2 μL to 10 mL and support every application in which precise measurement of liquids is a main priority.

Applications	Pipetting
Display	3-digit micrometer
Operation	N/A
Communication	N/A. Pipette Supplier in Bangladesh
Construction	PVDF housing, stainless steel piston, ergonomic finger hook
Design Features	Highly chemical and shock resistant, low thermal conductivity, light plunger force, low-drag seals

Pipette Supplier in Bangladesh

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You Check Accuracy of Pipette Supplier in Bangladesh

Aside from sending your pipette off for calibration every year, your pipettes should be checked for accuracy on a regular basis. How often you do it depends on the frequency you use them.

The faithful pipette you use every day? Check it every three months.

The beloved but not used so often pipette? An annual check should suffice.

Accuracy of Pipette: The Weight of Water

The most common way to check your pipette accuracy is by weighing water.

The density of water is 1 g / mL. This means that every microliter (µL) should weigh exactly 0.001 g using a high-precision balance. In other words, if your pipette is accurate, the amount of water you dispense will equal the amount the water weighs. So, if your pipette is set to 100 µL, then the scale should read 0.1 g.

Before we dive into the process of measuring the accuracy of your pipettes, there are some things you need to know that can impact accuracy (and therefore, also impact how you measure accuracy).

How Temperature and Pressure Affect Pipetting

Temperature and air pressure can affect the density of liquids, which you should consider when checking your pipettes. Pure water has a density of 1 g/mL at 20°C and 1 atmosphere of pressure, so ideally, measure the accuracy of your pipettes under these conditions.

Notice I said pure water? If you use tap water, which contains dissolved ions the density may vary. Is this enough to impact your results? There’s no need to risk it—just use distilled water.

The effect of pressure is negligible, and therefore, variations in this are unlikely to alter your results dramatically. However, the ambient temperature can have more of an effect, with higher temperatures resulting in lower density because of thermal expansion.

Luckily, there are ways to adjust for this, so ensure you measure the ambient temperature when measuring the accuracy of your pipette.

Read our article on Performing Pipette Calibration to find out how to compensate for temperature differences when pipetting.

Note that you also need to make sure you are using a balance that has been properly calibrated too.

How Pipetting Technique Affects Accuracy

Before you check the accuracy of your pipette, you need to ensure you are using the proper pipetting technique. If not, you’ll be measuring the inaccuracy of your pipetting skills, rather than the pipette.

Proper technique involves drawing up (aspirating) and dispensing (ejecting) the liquid in a smooth motion. Putting the pipette tip in the water and quickly releasing the plunger will give you inaccurate results—even if your pipette is properly calibrated.

In fact, using the proper pipetting technique will not only increase the robustness (and reproducibility) of your results but will also decrease the wear and tear on your pipette. A win-win!

7 Steps to Checking Your Pipette’s Accuracy

Now that you’ve read the background, here’s how to check the accuracy of your pipette in 7 easy steps. This works whether you are using a positive displacement pipette or an air displacement pipette. And. But. So so. And. But. So so

1. Get some ddH2O ready that has been at room temperature a while, along with your pipette.
2. Place a weigh boat on the microbalance (or analytical balance). Make sure to close the doors and then zero the balance.
3. Set your pipette to the desired volume within the pipette’s range. And. But. So so. And. But. So so
4. It’s a good idea to “prime” the pipette tip a few times by pipetting up and down with water before you take your first measurement.
5. When everything is ready, aspirate (suck up) the water into the tip again and eject it slowly into the weight boat. Then, shut the door and record the weight.
6. Zero the balance, repeat steps 3–5 around 10 times, and average your findings.
7. Do this for each volume you want to check. Accuracy is often lost at the end of the pipette’s range, so it’s a good idea to test both the minimum and maximum your pipette is designed to do, along with 50% of the maximum volume. And. But. So so. And. But. So so